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blunt end cloning vector pjet1 2  (Addgene inc)


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    Structured Review

    Addgene inc blunt end cloning vector pjet1 2
    Blunt End Cloning Vector Pjet1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1/NHR1-Flag+pcDNA3%2E1+(Plasmid+%2317315)/pmc12804166-56-8-12
    Average 94 stars, based on 2 article reviews
    blunt end cloning vector pjet1 2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: G Protein Gα q Subunits Engage Targets in the Nucleus Involved in Chromatin Remodeling and Gene Expression.
    Article Snippet: .. The following LgBiT constructs—all in in pcDNA3.1(+)—were obtained from Addgene: HAGq-LgBiT (#134360), HA-Gi1-LgBiT (#134342), and HA-Gs-LgBiT (#134362). ..

    Article Title: Peptide‑based therapeutics targeting the SLC39A14‑PIWIL2 fusion in hepatocellular carcinoma.
    Article Snippet: The SNU398 (Seoul, Republic of Korea; Cat# 00398_ SNU-398, RRID: CVCL_0077), SNU449 (KCLB Cat# 00449_SNU-449, RRID: CVCL_0454), HepG2 (KCLB Cat# 88065_HepG2, RRID: CVCL_0027), and Huh7 (KCLB Cat# 60104_Huh7, RRID: CVCL_0336) cell lines were purchased from Korean cell line bank and maintained in DMEM, MEM, or RPMI-1640 (Gibco BRL, Grand Island, NY) supplemented with 10% FBS and 1% antibiotics, at 37 °C in a 5% CO2 incubator. .. PIWIL2, PIWIL2 without exon 1 (X1-PIWIL2) and SLC39A14-PIWIL2 constructs (tPIWIL2) were cloned into PCDNA3.1 (RRID: Addgene_70219) or C-terminal 3xFLAG-tagged PCDNA3.1 (RRID: Addgene_208616) using the In-Fusion cloning method (Clontech, Mountain View, CA). .. PCR products were amplified with CloneAmp HiFi PCR Premix (TAKARA, Tokyo, Japan) and specific primers (Table S1), followed by insertion into PCDNA3.1 plasmid using HindIII and ApaI.

    Article Title: Peptide‑based therapeutics targeting the SLC39A14‑PIWIL2 fusion in hepatocellular carcinoma
    Article Snippet: The SNU398 (Seoul, Republic of Korea; Cat# 00398_SNU-398, RRID: CVCL_0077), SNU449 (KCLB Cat# 00449_SNU-449, RRID: CVCL_0454), HepG2 (KCLB Cat# 88065_HepG2, RRID: CVCL_0027), and Huh7 (KCLB Cat# 60104_Huh7, RRID: CVCL_0336) cell lines were purchased from Korean cell line bank and maintained in DMEM, MEM, or RPMI-1640 (Gibco BRL, Grand Island, NY) supplemented with 10% FBS and 1% antibiotics, at 37 °C in a 5% CO2 incubator. .. PIWIL2, PIWIL2 without exon 1 (X1-PIWIL2) and SLC39A14-PIWIL2 constructs (tPIWIL2) were cloned into PCDNA3.1 (RRID: Addgene_70219) or C-terminal 3xFLAG-tagged PCDNA3.1 (RRID: Addgene_208616) using the In-Fusion cloning method (Clontech, Mountain View, CA). .. PCR products were amplified with CloneAmp HiFi PCR Premix (TAKARA, Tokyo, Japan) and specific primers (Table S1), followed by insertion into PCDNA3.1 plasmid using HindIII and ApaI.

    Article Title: CD3ɛ Nanobody‐Engineered Extracellular Vesicles Driving In Vivo Generation of TCE‐secreting CAR‐Ts for Solid Tumor Therapy With Memory Response and Minimal Immunogenicity
    Article Snippet: .. These constructs were cloned into the pcDNA3.1 (Addgene) backbone with an EF‐1α promoter at the EcoRI/XbaI cloning site. .. Subsequently, these plasmids were subjected to the synthesis of linearized transgenes by polymerase chain reaction (PCR) through the following conditions: 35 cycles at 98°C for 20 s, 72°C for 30 s, and 72°C for 3.5 min) using HiFi KAPA DNA polymerase (Roche).

    Clone Assay:

    Article Title: Peptide‑based therapeutics targeting the SLC39A14‑PIWIL2 fusion in hepatocellular carcinoma.
    Article Snippet: The SNU398 (Seoul, Republic of Korea; Cat# 00398_ SNU-398, RRID: CVCL_0077), SNU449 (KCLB Cat# 00449_SNU-449, RRID: CVCL_0454), HepG2 (KCLB Cat# 88065_HepG2, RRID: CVCL_0027), and Huh7 (KCLB Cat# 60104_Huh7, RRID: CVCL_0336) cell lines were purchased from Korean cell line bank and maintained in DMEM, MEM, or RPMI-1640 (Gibco BRL, Grand Island, NY) supplemented with 10% FBS and 1% antibiotics, at 37 °C in a 5% CO2 incubator. .. PIWIL2, PIWIL2 without exon 1 (X1-PIWIL2) and SLC39A14-PIWIL2 constructs (tPIWIL2) were cloned into PCDNA3.1 (RRID: Addgene_70219) or C-terminal 3xFLAG-tagged PCDNA3.1 (RRID: Addgene_208616) using the In-Fusion cloning method (Clontech, Mountain View, CA). .. PCR products were amplified with CloneAmp HiFi PCR Premix (TAKARA, Tokyo, Japan) and specific primers (Table S1), followed by insertion into PCDNA3.1 plasmid using HindIII and ApaI.

    Article Title: A novel stromal cell source of RANKL during sustained osteoclast activation in disuse osteoporosis
    Article Snippet: figure legendMechanical unloading induced by spiral wire immobilization (SWI) leads to disuse osteoporosis.. During this process, osteoclastic bone resorption is persistently enhanced, with a brief reduction in osteoblastic activity © 2026 The Authors.. The Journal of Physiology © 2026 The Physiological Society.

    Article Title: Peptide‑based therapeutics targeting the SLC39A14‑PIWIL2 fusion in hepatocellular carcinoma
    Article Snippet: The SNU398 (Seoul, Republic of Korea; Cat# 00398_SNU-398, RRID: CVCL_0077), SNU449 (KCLB Cat# 00449_SNU-449, RRID: CVCL_0454), HepG2 (KCLB Cat# 88065_HepG2, RRID: CVCL_0027), and Huh7 (KCLB Cat# 60104_Huh7, RRID: CVCL_0336) cell lines were purchased from Korean cell line bank and maintained in DMEM, MEM, or RPMI-1640 (Gibco BRL, Grand Island, NY) supplemented with 10% FBS and 1% antibiotics, at 37 °C in a 5% CO2 incubator. .. PIWIL2, PIWIL2 without exon 1 (X1-PIWIL2) and SLC39A14-PIWIL2 constructs (tPIWIL2) were cloned into PCDNA3.1 (RRID: Addgene_70219) or C-terminal 3xFLAG-tagged PCDNA3.1 (RRID: Addgene_208616) using the In-Fusion cloning method (Clontech, Mountain View, CA). .. PCR products were amplified with CloneAmp HiFi PCR Premix (TAKARA, Tokyo, Japan) and specific primers (Table S1), followed by insertion into PCDNA3.1 plasmid using HindIII and ApaI.

    Article Title: Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy
    Article Snippet: .. A full-length mouse Prdm16 cDNA cloned into pcDNA3.1 was obtained from addgene (#15503). ..

    Article Title: CD3ɛ Nanobody‐Engineered Extracellular Vesicles Driving In Vivo Generation of TCE‐secreting CAR‐Ts for Solid Tumor Therapy With Memory Response and Minimal Immunogenicity
    Article Snippet: .. These constructs were cloned into the pcDNA3.1 (Addgene) backbone with an EF‐1α promoter at the EcoRI/XbaI cloning site. .. Subsequently, these plasmids were subjected to the synthesis of linearized transgenes by polymerase chain reaction (PCR) through the following conditions: 35 cycles at 98°C for 20 s, 72°C for 30 s, and 72°C for 3.5 min) using HiFi KAPA DNA polymerase (Roche).

    Article Title: Compositions comprising circular polyribonucleotides and uses thereof
    Article Snippet: .. GFP (SEQ ID NO: 2) is cloned into an expression vector, e.g. pcDNA3.1(+) (Addgene) (SEQ ID NO: 6). ..

    Cloning:

    Article Title: Peptide‑based therapeutics targeting the SLC39A14‑PIWIL2 fusion in hepatocellular carcinoma.
    Article Snippet: The SNU398 (Seoul, Republic of Korea; Cat# 00398_ SNU-398, RRID: CVCL_0077), SNU449 (KCLB Cat# 00449_SNU-449, RRID: CVCL_0454), HepG2 (KCLB Cat# 88065_HepG2, RRID: CVCL_0027), and Huh7 (KCLB Cat# 60104_Huh7, RRID: CVCL_0336) cell lines were purchased from Korean cell line bank and maintained in DMEM, MEM, or RPMI-1640 (Gibco BRL, Grand Island, NY) supplemented with 10% FBS and 1% antibiotics, at 37 °C in a 5% CO2 incubator. .. PIWIL2, PIWIL2 without exon 1 (X1-PIWIL2) and SLC39A14-PIWIL2 constructs (tPIWIL2) were cloned into PCDNA3.1 (RRID: Addgene_70219) or C-terminal 3xFLAG-tagged PCDNA3.1 (RRID: Addgene_208616) using the In-Fusion cloning method (Clontech, Mountain View, CA). .. PCR products were amplified with CloneAmp HiFi PCR Premix (TAKARA, Tokyo, Japan) and specific primers (Table S1), followed by insertion into PCDNA3.1 plasmid using HindIII and ApaI.

    Article Title: Peptide‑based therapeutics targeting the SLC39A14‑PIWIL2 fusion in hepatocellular carcinoma
    Article Snippet: The SNU398 (Seoul, Republic of Korea; Cat# 00398_SNU-398, RRID: CVCL_0077), SNU449 (KCLB Cat# 00449_SNU-449, RRID: CVCL_0454), HepG2 (KCLB Cat# 88065_HepG2, RRID: CVCL_0027), and Huh7 (KCLB Cat# 60104_Huh7, RRID: CVCL_0336) cell lines were purchased from Korean cell line bank and maintained in DMEM, MEM, or RPMI-1640 (Gibco BRL, Grand Island, NY) supplemented with 10% FBS and 1% antibiotics, at 37 °C in a 5% CO2 incubator. .. PIWIL2, PIWIL2 without exon 1 (X1-PIWIL2) and SLC39A14-PIWIL2 constructs (tPIWIL2) were cloned into PCDNA3.1 (RRID: Addgene_70219) or C-terminal 3xFLAG-tagged PCDNA3.1 (RRID: Addgene_208616) using the In-Fusion cloning method (Clontech, Mountain View, CA). .. PCR products were amplified with CloneAmp HiFi PCR Premix (TAKARA, Tokyo, Japan) and specific primers (Table S1), followed by insertion into PCDNA3.1 plasmid using HindIII and ApaI.

    Article Title: CD3ɛ Nanobody‐Engineered Extracellular Vesicles Driving In Vivo Generation of TCE‐secreting CAR‐Ts for Solid Tumor Therapy With Memory Response and Minimal Immunogenicity
    Article Snippet: .. These constructs were cloned into the pcDNA3.1 (Addgene) backbone with an EF‐1α promoter at the EcoRI/XbaI cloning site. .. Subsequently, these plasmids were subjected to the synthesis of linearized transgenes by polymerase chain reaction (PCR) through the following conditions: 35 cycles at 98°C for 20 s, 72°C for 30 s, and 72°C for 3.5 min) using HiFi KAPA DNA polymerase (Roche).

    Plasmid Preparation:

    Article Title: A novel stromal cell source of RANKL during sustained osteoclast activation in disuse osteoporosis
    Article Snippet: figure legendMechanical unloading induced by spiral wire immobilization (SWI) leads to disuse osteoporosis.. During this process, osteoclastic bone resorption is persistently enhanced, with a brief reduction in osteoblastic activity © 2026 The Authors.. The Journal of Physiology © 2026 The Physiological Society.

    Article Title: Notch mediates non-regenerative alveolar repair after staphylococcal lung injury
    Article Snippet: Plasmids were amplified and purified using an EndoFree Plasmid Maxi Kit (Qiagen). .. Plasmids included a Notch cleavage reporter (12XCSL-DsRedExpressDL, Addgene #47683); pcDNA3.1 and PS1 in pcDNA3.1 (PS1-OE, gifts of Dr. Alison Goate, Icahn School of Medicine at Mount Sinai); and GFP and Notch1 TMD-GFP each in the pTwist CMV mammalian expression vector (Twist Biosciences). siRNA included SMARTPool ON-TARGETplus siRNA against mouse presenilin1 and mouse non-targeting pool siRNA and was purchased from Dharmacon and reconstituted in molecular grade RNAse-free water (Dharmacon) according to the manufacturer’s instructions. .. Using our established methods ( , ), we complexed plasmids with freshy extruded unilamellar liposomes (20 μg/μL; 100 nm pore size; DOTAP; Avanti Lipids) in sterile Opti-MEM (Gibco).

    Expressing:

    Article Title: Compositions comprising circular polyribonucleotides and uses thereof
    Article Snippet: .. GFP (SEQ ID NO: 2) is cloned into an expression vector, e.g. pcDNA3.1(+) (Addgene) (SEQ ID NO: 6). ..

    Article Title: Notch mediates non-regenerative alveolar repair after staphylococcal lung injury
    Article Snippet: Plasmids were amplified and purified using an EndoFree Plasmid Maxi Kit (Qiagen). .. Plasmids included a Notch cleavage reporter (12XCSL-DsRedExpressDL, Addgene #47683); pcDNA3.1 and PS1 in pcDNA3.1 (PS1-OE, gifts of Dr. Alison Goate, Icahn School of Medicine at Mount Sinai); and GFP and Notch1 TMD-GFP each in the pTwist CMV mammalian expression vector (Twist Biosciences). siRNA included SMARTPool ON-TARGETplus siRNA against mouse presenilin1 and mouse non-targeting pool siRNA and was purchased from Dharmacon and reconstituted in molecular grade RNAse-free water (Dharmacon) according to the manufacturer’s instructions. .. Using our established methods ( , ), we complexed plasmids with freshy extruded unilamellar liposomes (20 μg/μL; 100 nm pore size; DOTAP; Avanti Lipids) in sterile Opti-MEM (Gibco).



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